Penetapan Keragaman Genetik Nilam (Pogostemon SP.) Hasil Fusi Protoplas Dengan Teknik Rapd. Yang Nuryani • Otih Rostiana • Cheppy Syukur. Pengembangan Metode Fusi Protoplas dengan Tingkat Keberhasilan Pembentukan Fusan 25% untuk Memperoleh Tanaman Triploid Jeruk Garut Tanpa Biji. Request PDF on ResearchGate | Karakterisasi Dan Identifikasi Molekuler Fusan Hasil Fusi Protoplas Interspesies Chlorella pyrenoidosa dan.

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Result showed that Java patchouli Girilaya contained phenols and lignin respectively DNA of the plant materials used were extracted by using the modified method of orozcocastulo et al.

Results showed that index of similarities of the amplified DNA from 5 random primers ranged from 0. Somatic hybridization by fusion technique is a breakthrough technique that allows the plant generated by an increased level ploidy. The second group was then classified into 2 minor groups.

Abstract Tangerine is a citrus seed Indonesia and including a true species of the genus Citrus. The best enzyme formulations for the isolation of fudi from diploid callus and leaves in vitro and protoplast Haploid pollen is macerozim 0.

The strategy chosen to assemble the triploid tangerine because the technique of protoplast isolation, fusion and regeneration of tangerine with diploid level has been mastered ploidy.

Abstract Evaluation prptoplas phenols and lignin in the root of hybrid patchouli Pogostemon cablin Benth. Sidikalang and TT 75 were tested for their genetic variability and relationship.


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The genetic relationship among the somatic hybrids were estimated by using the index of similarity to perform genctical matrix and dendogram. Metadata Show full item record. Haploid cell population repeatedly sub cultured every 4 weeks in a double layer media: The somatic hybrids and their parental plants subjected to RAPD analyses were classified into 2 major groups, first, the parental group of Java patchouli and second, others parental plants, Aceh patchouli Sidikalang and TT 75and the somatic hybrids.

Pusat Penelitian dan Pengembangan Perkebunan Jln. Plants with triploid ploidy level usually have sterile fruit seedless. One of the problems faced in patchouli production is nematode infection Pratylenchus brachyurus.

Email this article Login required. First group consisted of somatic hybrids nos. OPH 19 and Abi Evaluation of phenols and lignin in the root of hybrid patchouli Protopplas cablin Benth. The plant material used for protplas production of diploid callus is a population of diploid cells induced from embryonic nucellar tangerine.

View original from ejurnal. Some features of this site may not work without it. Ihe level of protopplas of different clones of somatic hybrids lo nematode was approached by evaluating ihe content of phenols and lignin of the roots The study was conducted al the laboratory of the Research Institute for food Crop Biotechnology, Bogor in November Based on the distribution of phenols and lignin content.


This orange tastes prktoplas but relatively many seeds fruit seed.

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Language Select Language English Indonesian. JavaScript is disabled for your browser. In the fusion of Girilaya x Sidikalang. Improvement of the plant resistance to nematode in patchouli is dificult 10 be conducted through conventional method as the plant never llowcrs.

Meanwhile, from Ihe fusion of Girilaya, Tapak Tuan. Article Tools Print fuusi article. Download full text Bahasa Indonesia, 6 pages.

Fusi protoplas interspesifik aspergillus Spp

Ihe second group 9 clones contained high lignin. It has been found thai Ihe plain resistance lo nematode is closely related to phenols and lignin content of the root Therefore. Phenol and lignin content in the somatic hybrids were dilferenl in Ihe respective pairs. How to cite item.

Penetapan Keragaman Genetik Nilam (Pogostemon SP.) Hasil Fusi Protoplas Dengan Teknik Rapd – Neliti

Five random primers, OPD User Username Password Remember me. Industrial Crops Research Journal. Seedless citrus improvement can be done by manipulating the level ploidy.